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Specifications Tween(R)20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.Synonyms: Polyethylene glycol sorbitan monolaurate; Polyoxyethylenesorbitan monolaurateMDL Number: MFCD00165986Purity: >=40.0% (GC)Density: 1.095 g/mL at 25 C (lit.)Refractive Index: n20/D 1.468 (lit.)Application: TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins). TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.Legal Information: TWEEN is a registered trademark of Croda International PLCAcid Number: ≤2.2 mg/gCMC: 0.06 mM(20-25°C)Composition: lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)Density: 1.095 g/mL at 25°C(lit.)Description: non-ionicForm: viscous liquidHLB: 16.7Hydroxyl Value: 96-108 mg/gImpurities: ≤3.0% waterMol Wt: mol wt ~1228Quality Level: PREMIUMRefractive Index: n20/D 1.468(lit.)Suitability: complies for IR spectroscopyTransition Temp: cloud point 76°C
