SYBR Green PCR Master Mix, Everything needed for SYBR Green dye-based PCR amplification and detection in a convenient, single-tube format, Each

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Applied Biosystems™ SYBR™ Green PCR Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.SYBR™ Green I dye detects double-stranded DNA, so specific probes are not requiredAmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performancedUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylaseProprietary buffer enhancements ensure performance and reliabilityPremixed components stored at 2° to 8°C significantly reduce assay setup timeMaximum Flexibility and Convenience:Applied Biosystems™ SYBR™ Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are requiredSYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCRThe hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivityPassive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuationsThis minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporationSYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.Store at -20°C