$ 441.60
Details
Lambda DNA digested with RsaI, 1.4% agarose, 113 cleavage sitesconventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.5'...G T▵A C...3'3'...C A▵T G...5'Conditions for 100% Activity 1X Buffer Tango: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA Incubate at 37°CStorage Buffer RsaI is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerolLigation and Recleavage After 50-fold overdigestion with RsaI, more than 95% of the DNA fragments can be ligated and recutMethylation Effect Dam: never overlaps —no effect Dcm: never overlaps —no effect CpG: may overlap —cleavage impaired EcoKI: never overlaps —no effect EcoBI: never overlaps —no effectThis product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.
