RNase H (5 U/µL), Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids, Each

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Ribonuclease H (Rnase H) specifically degrades the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.Does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA Quality Control:The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality testsSource:E.coli MRE-600 cellsMolecular Weight:18.4kDa monomerDefinition of Activity Unit:One unit of the enzyme catalyzes the formation of 1nmol of acid soluble products in 20 min. at 37°CEnzyme activity is assayed in the following mixture: 20mM Tris-HCl (pH 7.8), 40mM KCl, 8mM MgCl2, 1mM DTT, 24μM [3H]-poly(A)·poly(dT), 0.03mg/mL BSA, 4% (v/v) glycerol Storage Buffer:The enzyme is supplied in: 25mM HEPES-KOH (pH 8.0), 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.1mg/mL BSA and 50% (v/v) glycerol10X Reaction Buffer:200mM Tris-HCl (pH 7.8), 400mM KCl, 80mM MgCl2, 10mM DTTInhibition and Inactivation: Inhibitors: metal chelators, SH-blocking reagentsInactivated by heating at 65°C for 10 min. Recommended for:Removal of mRNA prior to synthesis of second strand cDNA (1)RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesisRemoval of the poly(A) sequences of mRNA after hybridization with oligo(dT) (2)Site-specific cleavage of RNA (3)Studies of in vitro polyadenylation reaction productsThis product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.