$ 228.00
Details
Thermo Scientific™ PvuII is available as a FastDigest enzyme which is used for rapid DNA digestion, more than 90% of the digested DNA fragments can be ligated and more than 95% of these sites can be recut at a 5′-termini concentration of 0.15 μM after a 10-fold overdigestion (5U/μg DNA x 2 hours) with PvuII.5'...C A G▵C T G...3'3'...G T C▵G A C...5'Conditions for 100% Activity1X Buffer G:10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSAIncubate at 37°CStorage BufferPvuII is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerolLigation and RecleavageAfter 10-fold overdigestion with PvuII, more than 90% of the DNA fragments can be ligated and more than 95% of these can be recutMethylation EffectsDam: never overlaps —no effectDcm: never overlaps —no effectCpG: never overlaps —no effectEcoKI: never overlaps —no effectEcoBI: may overlap —effect not determinedDigestion of Agarose-embedded DNAMinimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours Note:Greater than 15-fold overdigestion with PvuII may result in star activity.
