OPTIZYME DNA Polymerase I, Fisher BioReagents, Each

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OPTIZYME™ DNA Pol I is a template-dependent DNA Polymerase that catalyzes 5'->3' DNA synthesis, and exhibits 3'->5'-exonuclease, 5'->3' exonuclease and RNase H activity.Applications and Features: OPTIZYME DNA Polymerase I labels DNA by nick translation in conjunction with DNase I.Second-strand cDNA synthesis in conjunction with RNase H.Incorporation of modified nucleotides (e.g., biotin-, digoxigenin-, aminoallyl-, fluorescently-labeled nucleotides).Active in buffers for OPTIZYME restriction enzymes and reverse transcriptases.Supplied with: 10X OPTIZYME DNA Pol I Buffer: 500mM Tris-HCl (pH 7.5 at 25°C), 100mM MgCl2, 10mM DTTSource: E. Coli cells with a cloned polA gene for E.ColiQuality Control: Endodeoxyribonuclease-free