NEBNext Microbiome DNA Enrichment Kit – 24 reactions, Each

Details

NEBNext Microbiome DNA Enrichment Kit - 24 rxns. The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment. The Kit uses a simple and fast magnetic bead-based method to selectively bind and remove CpG-methylated host DNA. Application of a magnetic field then pulls out the CpG-methylated (eukaryotic) DNA, leaving the non-CpG-methylated (microbial) DNA in the supernatant. If desired, the host DNA captured in the magnetic bead pellet can be eluted, and a protocol is provided for this. This Kit is suitable for a wide range of sample types, including samples with high levels of contaminating host DNA, and is compatible with downstream applications including next generation sequencing on all platforms, qPCR and end-point PCR.