MUC-1 Ab-5 Hamster Monoclonal Antibody, MUC-1 Ab-5, Hamster Monoclonal Antibody provides accurate, reproducible results, Each

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Muc1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed in >90% breast carcinomas and metastases. In normal mammary gland it is expressed in apical surface of glandular epithelium. In breast cancer Muc1 is overexpressed; is underglycosylated and the apical localization is lost. Muc1 is transcribed as a larger precursor which is cleaved to form a larger mucin like subunit (265-400kDa) and a smaller subunit (14-28kDa) noncovalently associated with each other. Transgenic Muc1 has been shown to associate with all four erbB receptors and localize with erbB1 (EGFR) in lactating glands1. Muc1 can act as a ligand for ICAM-1 on HUVEC cells; it can bind beta-catenin, GSK3beta and it associates with Grb2-SOS upon phosphorylation. Host Species: HamsterClone: MH1 (CT2)Isotype: IgGSpecies Reactivity: Human and Mouse. Others not tested.Epitope: aa 239-255Immunogen: A synthetic peptide corresponding to aa 239-255 (SSLSYTNPAVAATSANL) from the cytoplasmic tail of MUC-1Molecular Weight: 14-28kDaPositive Control: MCF-7 cells; Breast CACellular Localization: Cytoplasmic and membrane Recommended for:Immunohistochemistry (Formalin/paraffin)Western BlottingImmunofluorescenceImmunoprecipitationFlow Cytometry Note:USA: RUO; Int'l: RUO