MssI (PmeI) (5 U/µL), The MssI (PmeI) restriction enzyme recognizes GTTT^AAAC sites and cuts best at 37°C in B buffer (Isoschizomers: PmeI), Each

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Lambda DNA digested with MssI (PmeI), 0.7% agarose, 2 cleavage sitesconventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.5'...G T T T▵A A A C...3'3'...C A A A▵T T T G...5'Conditions for 100% Activity: 1X Buffer B: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/mL BSA Incubate at 37°CStorage Buffer: MssI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerolMethylation Effects: Dam: never overlaps —no effect Dcm: never overlaps —no effect CpG: may overlap —no effect EcoKI: may overlap —blocked EcoBI: never overlaps —no effectDigestion of Agarose-embedded DNA: Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hour