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Specifically detects TPM3 clone: TC22-4 in Human samples, and it is validated for ELISA, Flow Cytometry, Immunohistochemistry, Western BlottingTropomyosins are dimmers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. The human TPM3 gene (Gene ID 7170; also known as TM3, TM5, TRK, CFTD, NEM1, TM-5, TM30, CAPM1, TM30nm, TPMsk3, hscp30, HEL-189, HEL-S-82p, OK/SW-cl.5) encodes seven tropomyosin alpha-3 chain isoforms (UniProt P06753; also known as Tropomyosin-3) as a result of alternative splicing. Spliced variants 1, 2, 4, and 5 are also known as the skeletal muscle, TM30nm, TC22, and hTM5 isoform, respectively. The TC22 and the hTM5 variants are identical in sequence other than the 25 amino acids at their C-terminal end. TC22 is expressed in 100% of colorectal carcinomas, but is not expressed in normal colon epithelial cells. The anticarcinogenic potency of sulfasalazine (SASP) and its active metabolite 5-aminosalicylate (5-ASA, mesalamine) in protecting ulcerative colitis (UC) patients from developing colorectal cancer is directly linked to their efficacy in reducing cellular expression of TC22. Likewise, siRNA-mediated TC22 downregulation is shown to significantly alter several critical carcinogenic pathways in the human colon adenocarcinoma LS180 cells.
