MEM α, nucleosides, Minimum Essential Medium is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells, Case of 6

Details

MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. This MEM is modified as follows: ribonucleosides, deoxyribonucleosides, phenol red, L-glutamine.Modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acidAvailable without nucleosides for use as a selection medium for DG44 and other DHFR-negative cellsMade with Earle's saltsContains no proteins, lipids, or growth factorsRequires supplementation, commonly with 10% Fetal Bovine Serum (FBS)Uses sodium bicarbonate buffer system (2.2g/L), and therefore requires 5-10% CO2 environment to maintain physiological pHSustainable packagingCell Culture, Mammalian Cell Culture