$ 103.07
Details
Protein A and Protein G Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose with native protein A or native protein G as ligand. Protein A and Protein G have high affinities for the Fc region of IgG antibodies and offer differing affinity characteristics across a broad range of mammalian IgG species.There are two protocols available for protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for for downstream applications such as electrophoresis and MS.Protein A and Protein G Mag Sepharose combine well-proven enrichment methods with the magnetic beads platform, which has ideal properties for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.Visible and dense Sepharose based magnetic beads with protein A or protein G ligand, easy to spot and collect bound target proteinNon-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergentsSimple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)Optimized capacity for enrichment/immunoprecipitation of target proteins - only low amounts of antibody neededMatrix: Paramagnetic, spherical, highly cross-linked agarose particles Ligand: Native protein A; Native protein G Binding capacity Protein A: 8-17mg human IgG/mL gel Binding capacity Protein G: 13-22mg human IgG/mL gelParticle size: 37-100μm Working temperature: Room temperature Storage solution: 20% ethanol Storage temperature: 4°C to 8°C
