Lentil Lectin Sepharose 4b Medium, Routinely Used In The Preparation And Purification Of A Number Of Glycoproteins And Carbohydrate-Containing Molecules, Each

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Lentil lectin coupled to sepharose 4b via cyanogen bromide activationlentil lectin is a metalloprotein, containing ca2 and mn2 , which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars retains binding in 1% deoxycholate; especially suited for purification of detergent-solubilized membrane proteinsligand concentration 2 mg lentil lectin/ml; binding capacity for porcine thyroglobulin approx. 16Mg/ml mediumaverage particle size 90μmstable in the ph range 3-10, and in the presence of all commonly used buffers (chelating agents and 8m urea to be avoided)has more equal affinity for α-d-mannose and α-d-glucose compared to con aorder infosupplied in 20% ethanol containing 0.9% Nacl, 1mm ca2 and 1mm mn2