Con A Sepharose 4b Medium, An Affinity Chromatography Media For Capture Of Glycosylated Biomolecules Including Glycoproteins And Glycolipids, Each

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Highly suited for isolation of cell surface glycoproteins from detergent-solubilized membranes.Con a coupled to sepharose, in a 4% agarose matrix via cyanogen bromide activationbinds molecules that contain α-d-mannose, α-d-glucose, and sterically related residues with available c-3, c-4, or c-5 hydroxyl groupsgroup-specific adsorbent for molecules containing sugarsconcentration of immobilized con a, approx. 13Mg/ml medium; binding capacity for porcine thyroglobulin, approx. 30Mg/ml mediumstable in the ph range 4 to 9, and in the presence of all commonly used buffersaverage particle size: 90μmdelivered in 0.1M acetate buffer, ph 6.0, Containing 1m nacl, 1mm cacl2, 1mm mgcl2, 1mm mncl2, and 20% ethanol