$ 89.40
Details
Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.5'...T T T▵A A A...3'3'...A A A▵T T T...5'Conditions for 100% Activity: 1X Buffer Tango™: 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA Incubate at 37°CStorage Buffer: DraI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerolLigation and Recleavage: After 50-fold overdigestion with DraI, more than 95% of the DNA fragments can be ligated and recutMethylation Effects: Dam: never overlaps — no effect Dcm: never overlaps — no effect CpG: never overlaps — no effect EcoKI: may overlap — blocked EcoBI: never overlaps — no effectDigestion of Agarose-embedded DNA: Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hour
