CD84 Mouse anti-Human, APC, Clone: 2G7, eBioscience, Mouse Monoclonal Antibody, Each

Details

: The monoclonal antibody 2G7 reacts to CD84, a transmembrane protein of the Ig SLAM superfamily. Expression of CD84 is found on mature B cells, a subset of T cells (mainly CD4 CD45RA ), monocytes and platelets. CD84 is found on a percentage of human hematopoietic progenitor (CD34 ) cells. Signaling through CD84 via homotypic interactions involves phosphorylation and recruitment of SAP and EAT-2 leading to production of IFNgamma and T cell activation. Applications Reported: This 2G7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2G7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.CD84 is expressed on mature B cells and on B cell lines, including pre B cell lines, but not on plasma cell lines. Immunohistochemical studies demonstrated that CD84 strongly expressed on tissues macrophages. CD84 is also highly expressed on platelets and, at low levels on peripheral blood T cells. It is a highly glycosylated protein and belongs to immunoglobulin superfamily. It may play a role in leukocyte activation.