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Reacts with FcγRII (CD32), a 40 kDa, polymorphic transmembranous glycoprotein, highly glycosylated molecule (encoded by at least two genes), expressed on monocytes, granulocytes, platelets and B cells. Unlike FLI28.6, 3D3 mAb detected a polymorphic CD32 antigen expressed on B cells of all donors, but only on platelets, monocytes and granulocytes of some donors. The platelets from 3D3 donors respond to certain stimulatory mAb such as CD165 (clone SN2, Cat. No. 556050) and result in aggregation. On the other hand, the platelets from 3D3 negative donors do not form aggregates after stimulation. Individuals can be divided into two groups as responder and non-responder depending on expression, or non-expression, of 3D3. In comparison to 3D3, FLI8.26 detects a monomorphic CD32 antigen expressed on all human donors. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
