Daf-2 Diacetate, 205391-02-2, 250ug, Each

Details:

Daf-2 diacetate is a sensitive fluorescent indicator for the detection and bioimaging of no.{6360,6632} It is a cell-permeable derivative of daf-2. Upon entry into the cell, daf-2 diacetate is transformed into the less cell-permeable daf-2 by cellular esterases thus preventing loss of signal due to diffusion of the molecule from the cell. In the presence of oxygen, daf-2 reacts with no to yield the highly fluorescent triazolofluorescein (daf-2t). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nm, respectively.{6632} At neutral ph the detection limit for no is 2-5 nm. Daf-2 diacetate can be utilized in cells which produce small amounts of no, such as endothelial cells, as well as in cells which generate large amount of no, such as macrophages.{6360,6632} 205391-02-2