Benchmark Agarose LE, 500g

Details

This Benchmark Scientific® Agarose LE 500g is a highly purified agarose, suitable for a variety of molecular biology applications. It is refined using an advanced process that excludes the use of organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact. Agarose LE can be used for analysis of proteins and nucleic acids of various sizes (150bp to 6kbp). Its low EEO (<0.13-m) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO also provides a reduction in brand distortion (caused by counterflow) that can result from the presence of excessive, sulfate-rich negative ions. Agarose LE is widely used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion), and various blotting protocols. It is easily soluble, free of nucleases, and easy to use. It is highly transparent (forms a clear, colorless solution at 1g:100mL H O), and exhibits exceptionally low absorbtion of chemical staining agents. Pore size can be adjusted by simple modfications to the concentration ratio. Moisture: < 7%. EEO (electroendosmosis): < 0.13 -mr. Sulfate: < 0.20%. Gelling Temp. (at 1.5%): 36 /- 1.5C. Melting Temp. (at 1.5%): 88 /- 1.5C. Gel Strength (at 1.0%): 1200g/sq cm. Gel Strength (at 1.5%): 2500g/sq cm. DNase/RNase: None detected. Protease: None detected. Endonuclease: None detected. Size: 500g. Benchmark Scientific® #: A1705.