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Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, condensation of cytoplasm and nucleus, and internucleosomal cleavage of DNA. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from inner to outer leaflet of plasma membrane, thereby exposing PS to external cellular environment35-36 kDa Ca2 dependent phospholipid-binding protein that has high affinity for PSBinds to cells with exposed PSMay be conjugated to fluorochromes, such as fluorescein isothiocyanate (FITC), to serve as sensitive probeTypically used in conjunction with vital dye such as 7-Amino-ActinomycinStorage Buffer: Aqueous buffered solution containing ≤0.09% sodium azideFormat: PurifiedFlow cytometry, Blocking
