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Sulfides are naturally present in ground waters as a result of leaching from sulfur-containing mineral deposits. Surface waters do not usually contain high sulfide concentrations. Sulfides result from the decomposition of organic matter, from bacterial sulfate reduction under anaerobic conditions and from various chemical processes.The Methylene Blue MethodReferences: USEPA Methods for Chemical Analysis of Water and Wastes, Method 376.2 (1983). APHA Standard Methods, 23rd ed., Method 4500-S2- D-2000.CHEMetrics test kits measure total acid soluble sulfides and employ the methylene blue methodology. Sulfides react with dimethyl-p-phenylenediamine in the presence of ferric chloride to produce methylene blue. Results are expressed as ppm (mg/L) S.
