$ 340.22
Details
For some applications, the use of a combination of ECM proteins, such as Laminin (LM) and Fibronectin (HFN) or LM and attachment factors such as Poly-D-Lysine (PDL) or Poly-L-Orinthine (PLO) has been shown superior to the use of either alone. Corning BioCoat PDL/LM and PLO/LM Cultureware is suitable for culturing many different types of Peripheral Nervous System (PNS) and Central Nervous System (CNS) networks and is useful for promoting neural cell attachment and differentiation. Corning BioCoat LM/HFN Cultureware provides an in vitro environment that promotes cell attachment and extensive process formation.Applications include:Enhancement of neuronal cell attachment to plastic and glassPromotion of neurite outgrowthCulture of glial cells as a feeder layer for neuronsConstruction of neural cell model systems to study CNS function, development, and diseasesSource: PDL, synthetic (MW 75-150 kD) PLO, synthetic (MW 30-70 kD) Laminin, EHS mouse tumor Fibronectin, human plasmaNOTE: Source material tested for hepatitis B antigen and HIV-1 antibody.Quality ControlPDL/LM and PLO/LM tested for ability to initiate differentiation (neurite outgrowth) of NG-108 rat glioma/mouse neuroblastoma cellsLM/HFN tested for receptor agonist induced changes in intracellular calcium using Fluo-3A (a long wavelength fluorescent calcium indicator) in primary rat cortical neuron enriched culturesTested and found negative for bacteria and fungiStorage: 2° to 8°C. Do not freeze.
